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Literature summary extracted from

  • Otero, L.H.; Beassoni, P.R.; Lisa, A.T.; Domenech, C.E.
    Transition from octahedral to tetrahedral geometry causes the activation or inhibition by Znf2+ of Pseudomonas aeruginosa phosphorylcholine phosphatase (2010), Biometals, 23, 307-314.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.75 His-tagged version expressed in Escherichia coli BL21 Codonplus Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.75 Zn2+ at pH 7.4, even in the presence of Mg2+ Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.75 Mg2+ at pH 5.0 and pH 7.4 Pseudomonas aeruginosa
3.1.3.75 Zn2+ at pH 5.0 Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.75 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.75 immobilized metal ion affinity chromatography (Ni2+) Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.75 54.5
-
 substrate phosphocholine, with 2 mM Mg2+ Pseudomonas aeruginosa
3.1.3.75 110
-
 substrate p-nitrophenyl phosphate Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.75 p-nitrophenyl phosphate + H2O
-
 Pseudomonas aeruginosa p-nitrophenol + phosphate
-
 ?
3.1.3.75 phosphocholine + H2O
-
 Pseudomonas aeruginosa choline + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.75 phosphorylcholine phosphatase
-
 Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.75 0.00035
-
 Zn2+ at pH 7.4 Pseudomonas aeruginosa